Bioassays

Developing, designing, verifying and validating your biochemical assays with us will provide you with knowhow, and often with the ‘one-off’ original solution in the most cost efficient way.

ELISA
Designing, developing & validating sensitive and specific immunoassays

Binding assays
Including: fluorescence intensity, FRET, TRF, fluorescence polarization (FP) and alpha screen

Protein analysis

Including: SDS-PAGE, western blotting, Iso-electric focusing, etc.

Enzymatic assays
Kinetic analysis including determination of Km, Vm, mono-and multi-substrate reactions

SOP Writing

Writing of easy to follow SOPs to be validated in third part CRO or client GMP facilities

Release Tests

We have gained successful experience in developing release tests assays specially designed to meet the FDA and EMA regulatory authorities.

The developing process includes characterization of standard references, matrix effects evaluation, limits of detection (LOD) determination and evaluation of product stability

Cell Based

Smart Assays design, develop, verify and validate numerous cell based assays on commercial cell lines including:

Dual luciferase
Mammalian two hybrid
Potency / Proliferation
Activation /Apoptosis
Cell Viability, cytotoxicity,
anchorage-dependent cell death, and cellular senescence
Angiogenesis Assay
Autophagy
Cell Adhesion and attachment Cell migration: Chemotaxis, Haptotaxis, Transmigration, Wound healing
Macrophage activation assays (Phagocytosis, Oxidative burst assay)
Cytokine assays
Cell manipulation
Transient and stable transfections
Induction of cell differentiation
Cell cycle synchronization