Bioassays

Bioassays

Developing, designing, verifying and validating your biochemical assays with us will provide you with knowhow, and often with the ‘one-off’ original solution in the most cost efficient way.

 

ELISA
Designing, developing & validating sensitive and specific immunoassays

 

Binding assays
Including: fluorescence intensity, FRET, TRF, fluorescence polarization (FP) and alpha screen

 

Biophysical assays
Differential scanning fluorometry (DSF)

 

Protein analysis
Including: SDS-PAGE, western blotting, Iso-electric focusing, etc.

 

Enzymatic assays
Kinetic analysis including determination of Km, Vm, mono-and multi-substrate reactions

 

Free radicals analysis
We offer development services of biochemical methods, and samples testing of oxidation and antioxidants activity including:

  • ORAC

  • FRAP

  • TEAC

  • Total peroxides

  • Lipid peroxidation

  • Total phenolics content

Cell Based

Smart Assays design, develop, verify and validate numerous cell based assays on commercial cell lines including:

  • Dual luciferase

  • Mammalian two hybrid

  • Potency / Proliferation  

  • Activation /Apoptosis

  • Cell Viability, cytotoxicity, 

  • anchorage-dependent cell death, and cellular senescence

  • Angiogenesis Assay

  • Autophagy

  • Cell Adhesion and attachment Cell migration: Chemotaxis, Haptotaxis, Transmigration, Wound healing

  • Macrophage activation assays (Phagocytosis, Oxidative burst assay)

  • Cytokine assays

  • Cell manipulation

  • Transient and stable transfections

  • Induction of cell differentiation

  • Cell cycle synchronization

 

Drug Discovery

 

Developing HTS assays

Innovative and efficient development of  successful bioassays designed to be used in High Throughput Screening (HTS). Shortening of development & learning curves for new bioassays

 

ADME-Tox

Including caco-2 mono layer transport, Cyp 450 screening, Plasma protein binding, Tox assays

 

SOP Writing 

Writing of easy to follow SOPs to be validated in third part CRO or client GMP facilities 

 

Contact

smart@assays.co.il

 

Tel: +972 (0)77-300-1087

Mobil: +972 (0)54-7241-251

Location

3 Pinchas Sapir St.,

Weizmann Science Park

Ness Ziona,

7414003 ISRAEL

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