Smart-Assays offers a broad set of biochemical, biophysical, biological and histology techniques to meet the need for antibody characterization.
Sandwich or direct ELISA:
A solid phase heterogeneous assay to determine antibody-antigen interaction. In addition, homogenous
state-of-the-art methodologies such as TRF, HTRF and Alpha Screen can be employed to increase sensitivity.
Analysis of free cytotoxic molecule released from the ADC.
A traditional assay for characterization of specicity, MW and purity of antibodies.
Immuno-Specic identication, MW and purity of
Differential Scanning Fluorimetry (DSF):
Measures the thermal stability of a target protein by performing thermal denaturation curve in the presence of a fluorescent dye during functional characterization (Nature Protocol, 2007).
Drug-to-antibody ratio measurements using UV/VIS spectroscopic analysis.
Cell-based assay to determine antibody-antigen interaction.
Quantitative and qualitative analysis of antibody internalization into cells using fluorescent methods.
Cell-based assays to determine the cytotoxic eect of the ADC using colorimetric, fluorometric and luminesentic assays.
Immunohistochemical proling of antibodies using commercial available and customized Tissue Multi Arrays.