



Smart-Assays offers a broad set of biochemical, biophysical, biological and histology techniques to meet the need for antibody characterization.
Biochemical
Assays
Sandwich or direct ELISA:
A solid phase heterogeneous assay to determine antibody-antigen interaction. In addition, homogenous
state-of-the-art methodologies such as TRF, HTRF and Alpha Screen can be employed to increase sensitivity.
Competition ELISA:
Analysis of free cytotoxic molecule released from the ADC.
SDS-PAGE:
A traditional assay for characterization of specicity, MW and purity of antibodies.
Western-Blot:
Immuno-Specic identication, MW and purity of
antibodies/drugs.
Differential Scanning Fluorimetry (DSF):
Measures the thermal stability of a target protein by performing thermal denaturation curve in the presence of a fluorescent dye during functional characterization (Nature Protocol, 2007).
UV/VIS:
Drug-to-antibody ratio measurements using UV/VIS spectroscopic analysis.
ELISA:
Cell-based assay to determine antibody-antigen interaction.
Internalization:
Quantitative and qualitative analysis of antibody internalization into cells using fluorescent methods.
Cytotoxicity:
Cell-based assays to determine the cytotoxic eect of the ADC using colorimetric, fluorometric and luminesentic assays.
Tissue Distribution:
Immunohistochemical proling of antibodies using commercial available and customized Tissue Multi Arrays.
Biophysical
Assays
